EVALUATION OF SOME ANTAGONISTIC BACTERIA FOR THE CONTROL OF ALTERNARIA SOLANI (ELLIS AND MARTIN) JONES AND GROUT CAUSE OF EARLY BLIGHT DISEASE OF TOMATO (SOLANUM LYCOPERSICUM L.)
DOI:
https://doi.org/10.58622/2k51kr17Keywords:
Antagonistic Bacteria, Alternaria Solani, Solanum Lycopersicum L.Abstract
Bacteria are capable to produce a wide range of metabolites and having potential to produce antifungal compounds. The present study was conducted to examine the potential for antagonism of eight different bacterial strains, Bacillus subtilis, B. Staphylococcus aureus, B. thuringiensis, B. Lentimorbus, B. cereus, B. coagulans, Pseudomonas fluorescens and Rhizobium sp. against Alternaria solani. All isolates were subjected to preliminary tests against A. solani, i.e. screening tests, agar disc method, and slide culture method resulting antagonistic activity against A. solani were observed in mostly selected bacteria. Among the eight bacterial species, Bacillus subtilis and Pseudomonas fluorescens showed effective biological control against A. solani. On the other hand B. thuringiensis and B. coagulans reduced 50% growth in slide culture technique, while Bacillus subtilis and Pseudomonas fluorescens completely inhibited the spore germination of A. solani as compared to control. Similar tests were conducted in greenhouse and open field conditions where the treatment of Bacillus subtilis and Pseudomonas fluorescens were applied against A. solani, resulting both the bacterial strains significantly reduced the growth of A. solani and showed significant increase in number of leaves, length of stem, root length, fresh and dry weights of root and stem as compared to positive control. While maximum growth was found in negative control. However, Phenolic contents were found significantly greater under greenhouse conditions following infection with Alternaria solani and bacterial treatments in all plants as compared to -ve controls.
Bacteria are capable to produce a wide range of metabolites and having potential to produce antifungal compounds. The present study was conducted to examine the potential for antagonism of eight different bacterial strains, Bacillus subtilis, B. Staphylococcus aureus, B. thuringiensis, B. Lentimorbus, B. cereus, B. coagulans, Pseudomonas fluorescens and Rhizobium sp. against Alternaria solani. All isolates were subjected to preliminary tests against A. solani, i.e. screening tests, agar disc method, and slide culture method resulting antagonistic activity against A. solani were observed in mostly selected bacteria. Among the eight bacterial species, Bacillus subtilis and Pseudomonas fluorescens showed effective biological control against A. solani. On the other hand B. thuringiensis and B. coagulans reduced 50% growth in slide culture technique, while Bacillus subtilis and Pseudomonas fluorescens completely inhibited the spore germination of A. solani as compared to control. Similar tests were conducted in greenhouse and open field conditions where the treatment of Bacillus subtilis and Pseudomonas fluorescens were applied against A. solani, resulting both the bacterial strains significantly reduced the growth of A. solani and showed significant increase in number of leaves, length of stem, root length, fresh and dry weights of root and stem as compared to positive control. While maximum growth was found in negative control. However, Phenolic contents were found significantly greater under greenhouse conditions following infection with Alternaria solani and bacterial treatments in all plants as compared to -ve controls.
